Cellometer® K2 Image Cytometer
The K2 is a PC-based cell imager for automated 4x bright field and dual-channel fluorescent image capture and analysis. The K2 accurately performs cell count, size, viability, intensity, and population analysis and achieves count and viability measurements 10x faster than manual methods. This instrument is ideal for cells lines and primary cells in complex samples characterized by heavy debris, red blood cell contamination, low cell concentrations, and multiple cell types, including PBMCs, stem cells, cancer cells, splenocytes, hepatocytes, and whole blood. In addition, the K2 can easily perform cell-based assays such as Apoptosis, Cell Cycle, and GFP analysis.
The Cellometer K2 utilizes bright field imaging and dual-fluorescence imaging to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.
The Cellometer K2 is specifically optimized for analysis of primary cells from peripheral blood, cord blood, bone marrow, and other complex samples for use in a wide range of research areas, including nucleated cells for transplantation, PBMCs for immunology, splenocytes for vaccine development, stem cells for cellular therapy and tumor cell suspensions for oncology. Dual-color fluorescence allows for staining of live and dead nucleated cells, generating accurate viability results even in the presence of debris, platelets, and red blood cells. Accurate analysis of both 'messy' and 'clean' samples enables the K2 to evaluate samples at a variety of points throughout sample processing - from initial collection, to separation, to cryopreservation.
The dual-fluorescence AO/PI method utilizes nuclear staining dyes that bind to nucleic acids in the cell nucleus. Because most mature mammalian red blood cells do not contain nuclei, only live and dead mononuclear cells produce a fluorescent signal. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step. Red blood cells, platelets, and debris are not counted in the fluorescent channels.
No two cells are the same. With the Cellometer K2 Image Cytometer, cell morphology can be immediately viewed on-screen in the bright field image. Counted cells are indicated on-screen for further verification that cells in the sample are being imaged and analyzed properly. Bright field counted images can be viewed for basic cell counting and trypan blue viability. Fluorescent counted images indicating counted live and dead nucleated cells can be viewed for dual-fluorescence primary cell viability assays. Users can confirm that cells are counted correctly based on size and shape, that cells within clumps are being counted individually and that red blood cells, platelets, and debris are being excluded from results.