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ZytoFast® Products for CISH Analysis

Achieving Chromogenic in situ Hybridization Results in just 4 Hours!

The ZytoFast® products are designed for outstandingly fast detection and discrimination of human pathogen viruses, e.g.
HPV, EBV, CMV, and the determination of lymphocyte clonality by detecting Ig-k and Ig-l light chain RNA by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections and cell samples.

ZytoFast®: Outstandingly fast CISH
Optimized protocols and faster tissue penetration due to short oligonucleotide probes of the ZytoFast® system, make the ZytoFast® CISH procedure outstandingly fast.

Single color results can be achieved within just 4 hours, hands-on time is about 2 hours!

High Sensitivity and Specificity
All ZytoFast® probes are tagged using the unique ZytoFast® HighTag System providing improved signal intensity! High specificity without risk of cross-hybridizations is obtained due to optimized oligonucleotide probes.

Advantages of CISH

  • Simultaneous observation of tissue morphology and CISH signals
  • No risk of false positives due to mispriming or contamination as with PCR
  • Easy method comparable to IHC
  • No costly equipment needed
  • Ability to test archival specimens
  • High sensitivity and specificity

The ZytoFast® system uses oligonucleotide probes tagged with Biotin or Digoxigenin (1) which are detected using enzyme-conjugated antibodies or streptavidin targeting the tags (2). The enzymatic reaction of chromogenic substrates (3), e.g. BCIP/NBT or AEC, leads to the formation of strong colour precipitates that can be visualized by light microscopy.

Item No.DescriptionQuantityPrice
ZYT-T1113400
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0.4 ml ZytoFast CMV Probe Digoxigenin 20/Tests,Storage Cond:2-8deg,Delivery Cond:4deg, For RUO // 0,4 mL ZytoFast CMV Sonde digoxigénine 20 tests, entreposage:2-8deg, livraison:4deg, pour recherche seulementLogin to see pricing