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ZytoDot® Products for CISH Analysis
Reliable and Simple Detection of Genomic Alterations using Light Microscopy!
The ZytoDot® products are designed for the detection of aneuploidies and gene amplifications by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections, cell samples, blood or bone marrow smears, and metaphase chromosome spreads.
CISH: A reliable Alternative to FISH High concordance between CISH and FISH ranging from 92-100% has been shown by numerous international studies for ERBB2 amplification.
Advantages of CISH
Quick and easy interpretation of results comparable to IHC
Simultaneous observation of tissue morphology and CISH signals
Storage of slides at room temperature - CISH signals are permanent
No costly fluorescent microscope needed
High Signal-to-Noise Ratio The ZytoDot® probes are processed by the unique ZytoVision® Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed!
ZytoDot® Kits – Convenient Solutions For making CISH analysis reliable and user-friendly, all ZytoDot® CISH probes can be combined with the ZytoDot® CISH Implementation Kit (C-3018-40) which includes all necessary pretreatment solutions, wash buffers, antibodies, chromogenic substrates, counterstaining solution, mounting solution and a detailed protocol to perform successful CISH experiments. Additionally, for some major targets, complete kits including probes and all necessary reagents are available.
ZytoDot® 2C™ Products for CISH Analysis
2-Color CISH for the Detection of Genomic Alterations
The ZytoDot® 2C™ products are designed for the simultaneous detection of two different genomic targets by Chromogenic in situ Hybridization (CISH) in formalinfixed, paraffin-embedded tissue sections, cell samples, and blood or bone marrow smears. This two color system is especially useful for the differentiation of aneuploidies from gene amplifications, and the detection of deletions and translocations.
Advantages of ZytoDot® 2C™
Simultaneous observation of tissue morphology and CISH signals at 40x using light microscopy
Two targets detected simultaneously
High contrasting distinct red and green signals
Quick and easy interpretation of results comparable to IHC
Standardized and complete kits
No costly fluorescent microscope needed
ZytoDot® 2C™ Kits – Standardized Solutions For making CISH analysis reliable and user-friendly, complete ZytoDot® 2C™ kits are available for some major targets. These kits include a ZytoDot® 2C™ probe, all necessary pretreatment solutions, wash buffers, antibodies, chromogenic substrates, counterstaining and mounting solutions, and a detailed protocol.
For other targets, any separately available ZytoDot® 2C™ probe can be combined with ZytoDot® 2C™ Implementation Kits resulting in target specific kit solutions.
The ZytoDot® system uses Digoxigenin-labeled probes (1) which are, after blocking (2), detected using a Mouse-anti-Digoxigenin antibody (3). This antibody is detected by a polymerized HRP-Goat-anti-Mouse antibody (4). The enzymatic reaction of DAB (5) leads to the formation of strong permanent brown signals that can be visualized by light microscopy using a 40x objective.
ZytoDot® 2C™ System
The ZytoDot® 2C™ system uses DIGand DNP-labeled probe cocktails targeting different genomic sections (1) which are detected using a Mouse-anti-DIG/Rabbit-anti-DNP cocktail (2). These antibodies are detected by a unique cocktail of polymerized HRP-Goat-anti-Mouse/AP-Goat-anti-Rabbit antibodies (3). The enzymatic reaction of AP-Red (4) and HRP-Green 5 leads to the formation of strong permanent red respectively green signals that can be visualized by light microscopy using a 40x objective.
Reliable and Simple Detection of Genomic Alterations using Light Microscopy!
The ZytoDot® products are designed for the detection of aneuploidies and gene amplifications by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections, cell samples, blood or bone marrow smears, and metaphase chromosome spreads.
CISH: A reliable Alternative to FISH High concordance between CISH and FISH ranging from 92-100% has been shown by numerous international studies for ERBB2 amplification.
Advantages of CISH
Quick and easy interpretation of results comparable to IHC
Simultaneous observation of tissue morphology and CISH signals
Storage of slides at room temperature - CISH signals are permanent
No costly fluorescent microscope needed
High Signal-to-Noise Ratio The ZytoDot® probes are processed by the unique ZytoVision® Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed!
ZytoDot® Kits – Convenient Solutions For making CISH analysis reliable and user-friendly, all ZytoDot® CISH probes can be combined with the ZytoDot® CISH Implementation Kit (C-3018-40) which includes all necessary pretreatment solutions, wash buffers, antibodies, chromogenic substrates, counterstaining solution, mounting solution and a detailed protocol to perform successful CISH experiments. Additionally, for some major targets, complete kits including probes and all necessary reagents are available.
ZytoDot® 2C™ Products for CISH Analysis
2-Color CISH for the Detection of Genomic Alterations
The ZytoDot® 2C™ products are designed for the simultaneous detection of two different genomic targets by Chromogenic in situ Hybridization (CISH) in formalinfixed, paraffin-embedded tissue sections, cell samples, and blood or bone marrow smears. This two color system is especially useful for the differentiation of aneuploidies from gene amplifications, and the detection of deletions and translocations.
Advantages of ZytoDot® 2C™
Simultaneous observation of tissue morphology and CISH signals at 40x using light microscopy
Two targets detected simultaneously
High contrasting distinct red and green signals
Quick and easy interpretation of results comparable to IHC
Standardized and complete kits
No costly fluorescent microscope needed
ZytoDot® 2C™ Kits – Standardized Solutions For making CISH analysis reliable and user-friendly, complete ZytoDot® 2C™ kits are available for some major targets. These kits include a ZytoDot® 2C™ probe, all necessary pretreatment solutions, wash buffers, antibodies, chromogenic substrates, counterstaining and mounting solutions, and a detailed protocol.
For other targets, any separately available ZytoDot® 2C™ probe can be combined with ZytoDot® 2C™ Implementation Kits resulting in target specific kit solutions.
ZytoDot® System
The ZytoDot® system uses Digoxigenin-labeled probes (1) which are, after blocking (2), detected using a Mouse-anti-Digoxigenin antibody (3). This antibody is detected by a polymerized HRP-Goat-anti-Mouse antibody (4). The enzymatic reaction of DAB (5) leads to the formation of strong permanent brown signals that can be visualized by light microscopy using a 40x objective.
ZytoDot® 2C™ System
The ZytoDot® 2C™ system uses DIGand DNP-labeled probe cocktails targeting different genomic sections (1) which are detected using a Mouse-anti-DIG/Rabbit-anti-DNP cocktail (2). These antibodies are detected by a unique cocktail of polymerized HRP-Goat-anti-Mouse/AP-Goat-anti-Rabbit antibodies (3). The enzymatic reaction of AP-Red (4) and HRP-Green 5 leads to the formation of strong permanent red respectively green signals that can be visualized by light microscopy using a 40x objective.
https://www.zytovision.com/ZytoVision
ZytoDot® System
The ZytoDot® system uses Digoxigenin-labeled probes (1) which are, after blocking (2), detected using a Mouse-anti-Digoxigenin antibody (3). This antibody is detected by a polymerized HRP-Goat-anti-Mouse antibody (4). The enzymatic reaction of DAB (5) leads to the formation of strong permanent brown signals that can be visualized by light microscopy using a 40x objective.
ZytoDot® 2C™ System
The ZytoDot® 2C™ system uses DIGand DNP-labeled probe cocktails targeting different genomic sections (1) which are detected using a Mouse-anti-DIG/Rabbit-anti-DNP cocktail (2). These antibodies are detected by a unique cocktail of polymerized HRP-Goat-anti-Mouse/AP-Goat-anti-Rabbit antibodies (3). The enzymatic reaction of AP-Red (4) and HRP-Green 5 leads to the formation of strong permanent red respectively green signals that can be visualized by light microscopy using a 40x objective.
