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ZytoFast® PLUS Products for CISH Analysis
ZytoFast® PLUS for Increased Sensitivity!
The ZytoFast® PLUS products are designed for outstandingly fast and sensitive detection and discrimination of human pathogen viruses, e.g. HPV, EBV, CMV, and the determination of lymphocyte clonality by detecting Ig-k and Ig-l light chain RNA by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections and cell samples. The signal intensity of ZytoFast® probes is increased even more when using the ZytoFast ® PLUS Implementation Kits.
ZytoFast® PLUS – Outstandingly fast and sensitive CISH Depending on the time required for dewaxing and pretreatment of tissue sections, ZytoFast® PLUS protocols can be performed within approx. 4 hours! Thus, due to optimized protocols, the ZytoFast® PLUS method takes only slightly more time compared to ZytoFast ® protocols while being much more sensitive!
ZytoFast® PLUS – Flexibility that meets your Needs Several ZytoFast® PLUS CISH Implementation Kits using different enzyme/substrate combinations can be combined with any separately available Digoxigenin-labeled ZytoFast® probe to meet your preferences concerning the detection chemistry, counterstaining, and embedding. Each ZytoFast® PLUS CISH Implementation Kit includes a detailed protocol, all necessary reagents as well as positive and negative control probes for versatile use in DNA as well as RNA in situ hybridizations.
The ZytoFast® PLUS system uses Digoxigenin-labeled probes (1) which are detected using primary antibodies (2). These antibodies are detected by polymerized enzymeconjugated secondary antibodies (3). The enzymatic reaction of chromogenic substrates (4), e.g. NBT/BCIP or DAB, leads to the formation of strong color precipitates that can be visualized by light microscopy.
The ZytoFast® PLUS products are designed for outstandingly fast and sensitive detection and discrimination of human pathogen viruses, e.g. HPV, EBV, CMV, and the determination of lymphocyte clonality by detecting Ig-k and Ig-l light chain RNA by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections and cell samples. The signal intensity of ZytoFast® probes is increased even more when using the ZytoFast ® PLUS Implementation Kits.
ZytoFast® PLUS – Outstandingly fast and sensitive CISH Depending on the time required for dewaxing and pretreatment of tissue sections, ZytoFast® PLUS protocols can be performed within approx. 4 hours! Thus, due to optimized protocols, the ZytoFast® PLUS method takes only slightly more time compared to ZytoFast ® protocols while being much more sensitive!
ZytoFast® PLUS – Flexibility that meets your Needs Several ZytoFast® PLUS CISH Implementation Kits using different enzyme/substrate combinations can be combined with any separately available Digoxigenin-labeled ZytoFast® probe to meet your preferences concerning the detection chemistry, counterstaining, and embedding. Each ZytoFast® PLUS CISH Implementation Kit includes a detailed protocol, all necessary reagents as well as positive and negative control probes for versatile use in DNA as well as RNA in situ hybridizations.
The ZytoFast® PLUS system uses Digoxigenin-labeled probes (1) which are detected using primary antibodies (2). These antibodies are detected by polymerized enzymeconjugated secondary antibodies (3). The enzymatic reaction of chromogenic substrates (4), e.g. NBT/BCIP or DAB, leads to the formation of strong color precipitates that can be visualized by light microscopy.
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The ZytoFast® PLUS system uses Digoxigenin-labeled probes (1) which are detected using primary antibodies (2). These antibodies are detected by polymerized enzymeconjugated secondary antibodies (3). The enzymatic reaction of chromogenic substrates (4), e.g. NBT/BCIP or DAB, leads to the formation of strong color precipitates that can be visualized by light microscopy.
