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Celígo® Image Cytometry

Every Cell. Every Well.

The bench-top Celigo image cytometry system provides high-throughput whole-well imaging and quantitative data through image analysis in bright field and up to four fluorescent channels, for a wide variety of cell-based assays. It is routinely used to investigate adherent and suspension cells, 3D tumor spheroids and colonies of iPSC and cancer stem cells. It is compatible with microwell plates from 6 to 1536-well and T-flask formats.

The work-flow based intuitive software provides concurrent imaging and analysis; kinetic analysis such as time-lapse growth tracking and flow cytometry-like gating analysis and reporting of cell populations. Cell images of specific populations may be displayed with color overlays through a gating selection.

The Celigo product allows users to perform high-speed, fully automated imaging and quantification of a wide range of cell types across complex sample types. It enables an extensive menu of applications including label-free cell counting, confluence-based cell growth tracking, killing assays, apoptosis, cell cycle analysis, migration and invasion assays, as well as cellular assays for receptor internalization, protein expression and detection, phosphorylation and phagocytosis.




Whole-Well, High Resolution Images Acquired at High Speed

  • Proprietary optical design enables uniform illumination and consistent edge contrast
  • Image and count every cell in each well: 0 - 100,000 cells/96-well
  • 5 imaging channels with bright field and 4 fluorescent colors
  • Fast scanning for image acquisition and analysis with minimal plate movement ensuring minimal sample disruption
  • Accurately quantify cells and colonies with a non-invasive method
  • Ability to save experiment settings – quickly run the same assay on many plates without additional set up
  • Measure adherent cells without trypsinization
  • Powerful, easy-to-use image analysis software – accessible for everyone in the lab
  • System stitches multiple fields of view into a full resolution image
  • Easily integrates with robotic arms, plate stackers, automated incubators and liquid handlers
Software
  • Proprietary image acquisition and processing software
  • Powerful analysis software/Dell Precision computer
  • Windows 7
Illumination/Optics
  • 1 LED-based enhanced brightfield imaging channel with uniform well illumination
  • 4 LED-based fluorescent channels
  • Proprietary F-theta lens with superior well edge-to-edge contrast
  • Galvanometric mirrors for fast imaging of large areas
  • Large chip CCD camera (2024 x 2024 pixels)
  • 1, 2, 4 or 8 µm/pixel resolution
Fluorescent ChannelsChannelExcitationDichroicEmissionTypical Dyes
Blue377/50409470/22Hoechst, DAPI
Green483/32506536/40FITC, Calcein, GFP, AlexaFluor® 488
Red531/40593629/53R-PE, PI, Texas Red, AlexaFluor® 568
Far-Red628/40660688/31DRAQ5®, AlexaFluor® 647
Plate Compatibility
  • 6, 12, 24 48, 96, 384, 1536 well plates (black, white and clear wall plates)
  • T-25 and T-75 flasks
  • Slides and cell arrays plate profiles available upon request
High-Speed ImagingLess than 2 minutes per 384-well plate
Dimensions19.5"W x 16"H x 24"D (49.5cm x 40cm x 61cm)
Weight117 lbs. (53 kg)
Power Requirements110-220 VAC 50-60 Hz
Regulatory ComplianceCE marking
Item No.DescriptionQuantityPrice
NEX-200BFFL5C
Celigo S Imaging Cytometer - 5 Channels, Brightfield and Fluorescence // Cytomètre en image Celigo S - 5 canaux, champs clair et fluorescenceLogin to see pricing
NEX-200BFFLS
Celigo S Imaging Cytometer, Brightfield and Fluorescence // Cytomètre en image Celigo S, champs clair et fluorescenceLogin to see pricing