Cellometer® X1 & X2 Image Cytometers
The X1 and X2 are PC-based cell imagers for automated 10x bright field and either single channel (X1) or dual channel (X2) fluorescent image capture and analysis. Both instruments accurately perform size-based cell counting, viability, intensity, and population analysis and achieve those measurements in less than 60 seconds. The instruments' advanced image analysis software omits debris and is optimal for yeast, platelets and other small cells. The X1 and X2 are ideal for measuring yeast concentration and viability in brewing fermentations. In addition, lager and ale yeast vitality can be measured using the X2.
The Cellometer X2 utilizes bright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.
Fluorescent dyes that stain DNA can be used to identify nucleated cells in a mixed cell population. Acridine orange (AO) is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence when imaged with the Cellometer X2. Because mature mammalian red blood cells don't contain nuclei, only mononuclear cells produce a fluorescent signal. Fluorescent-positive nucleated cells are easily counted. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step.
Use of a fluorescent dye, such as propidium iodide (PI), is highly recommended for accurate viability analysis of cell samples containing debris. Propidium iodide (PI) is a nuclear staining (nucleic acid binding) dye that enters dead cells with compromised membranes. It stains all dead nucleated cells to generate red fluorescence. Because the Cellometer X2 can count dead cells in the red fluorescence channel, there is no interference from debris and live cells.
The Cellometer X2 Image Cytometer is specifically optimized for simple, 1-step determination of yeast concentration and viability. The Cellometer X2 is ideal for research laboratories and small and large breweries looking to automate their fermentation monitoring. Performance of the Cellometer X2 has been proven in the largest breweries in the U.S. Bright field images can be viewed to confirm cell morphology. Counted bright field images show counted cells within cell clumps. Fluorescent images show dead cells for confirmation of viability results. Analysis of both bright field and fluorescent images from each sample makes 1-step concentration & viability determination possible.